Awọn ọja
HEPES iṣu soda iyọ
Awọn itumọ ọrọ sisọ: HEPES sodium iyọ; 75277-39-3; HEPES hemisodium iyọ; 103404-87-1; Sodium 2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; iṣuu soda 2- [4- (2-hydroxyethyl). ) piperazin-1-yl] etanesulfonate; HEPES (sodium); C8H17N2NaO4S; 4- (2-Hydroxyethyl) piperazine-1-ethanesulfonic acid hemisodium iyọ; 1-Piperazineethanesulfonic acid, 4- (2-hydroxyethyl) -, iyọ monosodium; 4- (2-Hydroxyethyl) piperazine-1- ethanesulfonic acid iyọ iṣu soda; 4- (2-hydroxyethyl) piperazin-1-ylethanesulphonate; UNII-Z9FTO91O8A; Z9FTO91O8A; iṣuu soda; 2- [4- (2-hydroxyethyl) piperazin-1-yl] ethanesulfonate; DTXSID4044458; CHEBI: 4675 278-169-7; 1-Piperazineethanesulfonic acid, 4- (2-hydroxyethyl) -, iṣuu soda (1: 1); N- (2-Hydroxyethyl) piperazine-N'-2-ethanesulfonic acid, iyọ iṣuu soda; HEPES soda soda. iyọ,> = 99,5% (titration); iṣuu soda hepes; N- (2-Hydroxyethyl) piperazine-N'- (2-ethanesulfonic acid) iyọ iṣuu soda; MFCD00036463; HEPES, iyọ iṣuu soda; HEPES sodium iyọ, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-SNA iyọ iyọ; (1M); Tox21_302155; HB5187; N- (2-Hydroxyethyl) piperazine-N- (2-ethanesulfonicacid) hemisodiumsalt; HEPES iṣu soda iyọ, biokemika ite;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-06108Adium;36108Adium; N'-hydroxyethyl-N-piperazineethanesulfonate; iyo HEPES iṣuu soda, BioXtra,> = 99.0% (titration); HEPES sodium iyọ, Vetec (TM) reagent ite, 96%; W-104397; Q27120698; Sodium2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; 4- (2-Hydroxyethyl) -1-piperazineethanesulfonic acid sodium iyọ; 4- (2-hydroxyethyl) 1-piperazineethanesulfonic acid, monosodium iyọ; N- (2-Hydroxyethyl) piperazine-N'- (2-ethanesulfonic acid) sodiumsalt; N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium iyọ; HEPES sodium iyọ, BioPerformance Ifọwọsi, o dara fun aṣa sẹẹli, >> 99.5%
Ohun-ini Kemikali ti HEPES Sodium Salt
● Irisi / Awọ: funfun lulú
● Ipa oru: 0Pa ni 25 ℃
● Oju Iyọ: 234 °C
● PKA: 7.5 (ni 25 ℃)
● PSA:92.29000
● iwuwo:1.504[ni 20℃]
● LogP: -0.90190
● Ibi ipamọ otutu: Itaja ni RT.
● Kokoro.:Hygroscopic
● Solubility.: H2O: 1 M ni 20 °C, kedere, ti ko ni awọ
● Omi Solubility.: O jẹ tiotuka ninu omi.
● Awọn oluranlọwọ Idena Hydrogen: 1
● Awọn olugba Idena Hydrogen: 6
● Iwọn Idena Yiyi: 5
● Iwọn gangan: 260.08067248
● Iwọn Atomu Eru:16
● Àkópọ̀:272
Alaye ailewu
● Pitogram(s):
● Awọn koodu ewu:
● Awọn Gbólóhùn Aabo: 22-24/25
Wulo
Awọn kilasi Kemikali:Awọn ipawo miiran -> Awọn ifipamọ Biological
SILES Canonical:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Nlo:Ojutu iyọ iṣu soda HEPES dara fun awọn ẹkọ aṣa sẹẹli. O le ṣee lo ni igbaradi ti ojutu ifipamọ ti HEPES. O le ṣee lo bi ifipamọ lakoko awọn idanwo phosphorylation ni awọn fibroblasts permeabilized. O le ṣe lo lati ṣajọ ifipamọ itọsẹ ti o nipọn, ti a lo ninu ipinnu ti thyroxin ọfẹ ninu omi ara nipasẹ radioimmunoassay. Ojutu iyọ iṣu soda HEPES dara fun lilo ninu awọn ẹkọ aṣa sẹẹli. O le ṣee lo ni igbaradi ti ojutu ifipamọ ti HEPES. O le ṣee lo bi ifipamọ lakoko awọn idanwo phosphorylation ni awọn fibroblasts permeabilized. O le ṣe lo lati ṣajọ ifipamọ itọ-ọgbẹ ti o nipọn, ti a lo ninu ipinnu ti thyroxin ọfẹ ninu omi ara nipasẹ radioimmunoassay.
Alaye Ifihan
HEPES iṣu soda iyọ, ti a tun mọ ni 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium iyọ, jẹ fọọmu ti o wọpọ ti HEPES. O jẹ apapọ Organic zwitterionic ti o n ṣe bi aṣoju ifibọ ninu iwadii ti ẹkọ nipa ti ẹkọ ati kemikali.
Iyọ iṣu iṣuu soda HEPES nigbagbogbo ni a lo ni media asa sẹẹli ati awọn buffers ti ibi nitori agbara rẹ lati ṣetọju iwọn pH iduroṣinṣin ni ayika awọn ipo iṣe-iṣe (pH 7.2 - 7.6). O jẹ tiotuka pupọ ninu omi ati pe o ni eero ti o kere pupọ, ti o jẹ ki o dara fun awọn ohun elo lọpọlọpọ.
Fọọmu iyọ iṣuu soda ti HEPES jẹ ayanfẹ ni ọpọlọpọ awọn ohun elo bi o ṣe nmu solubility ati iduroṣinṣin, ni akawe si fọọmu acid ọfẹ. O wa ni iṣowo bi lulú kirisita funfun ti o le ni irọrun ni tituka ninu omi lati mura awọn ojutu ṣiṣẹ.
Awọn oniwadi nigbagbogbo lo iyo HEPES iṣuu soda ni awọn imọ-ẹrọ isedale molikula, aṣa sẹẹli, awọn idanwo enzymu, isọdi amuaradagba, ati awọn adanwo biokemika miiran nibiti iṣakoso pH ṣe pataki. Agbara ifipamọ ati ibaramu pẹlu awọn ọna ṣiṣe ti ibi jẹ ki o jẹ yiyan olokiki fun mimu awọn ipo aipe fun iṣẹ ṣiṣe ati iduroṣinṣin ti ọpọlọpọ awọn ohun elo ti ibi.
Ṣaaju lilo iyo HEPES iṣuu soda ni eyikeyi ohun elo kan pato, o ṣe pataki lati ṣe atunyẹwo awọn iwe-iwe, kan si awọn iṣeduro awọn olupese, ati mu ifọkansi ati iwọn pH ti o da lori awọn ibeere idanwo kan pato.
Ohun elo
Iyọ iṣu soda HEPES wa ohun elo ni ibigbogbo ni ọpọlọpọ awọn agbegbe iwadii ti isedale ati biokemika. Diẹ ninu awọn lilo bọtini rẹ pẹlu:
Asa sẹẹli:Iyọ iṣu iṣuu soda HEPES ni a ṣafikun nigbagbogbo si media asa sẹẹli lati ṣetọju pH iduroṣinṣin laarin sakani ti ẹkọ iṣe-ara ati pese agbegbe ti o dara fun awọn sẹẹli lati dagba ati siwaju.
Aṣoju ifipamọ:Iyọ iṣu soda HEPES nigbagbogbo ni iṣẹ bi oluranlowo ififunni ninu awọn buffers ti ibi ati awọn ojutu, pẹlu awọn idanwo henensiamu, ìwẹnumọ amuaradagba, ati awọn ilana isedale molikula. O ṣe iranlọwọ lati ṣetọju pH igbagbogbo nipasẹ kikoju awọn ayipada ninu acidity tabi alkalinity.
Electrophoresis: HEPES iyọ iṣuu soda ni a lo bi ifipamọ ni gel electrophoresis lati ṣetọju pH iduroṣinṣin lakoko ipinya ti awọn acids nucleic tabi awọn ọlọjẹ. O ṣe idiwọ awọn iṣipopada ni pH ti o le ni ipa lori ijira ati awọn ilana iyapa ti awọn ohun elo ninu jeli.
Iduroṣinṣin Amuaradagba: Iyọ iṣu soda HEPES ni a ṣafikun si awọn solusan amuaradagba lati ṣe iduroṣinṣin eto wọn ati ṣetọju iṣẹ ṣiṣe wọn. O ṣe iranlọwọ lati ṣetọju agbegbe pH ti o dara julọ pataki fun iduroṣinṣin amuaradagba ati iṣẹ ṣiṣe.
Iṣẹ iṣe Enzyme: Iyọ iṣu soda HEPES ni a lo bi ifipamọ ni awọn aati enzymatic lati ṣetọju pH ti o fẹ fun iṣẹ ṣiṣe henensiamu to dara julọ. O ṣe iranlọwọ rii daju pe awọn enzymu ṣiṣẹ ni ṣiṣe ti o ga julọ ati deede.
Aworan Cell Live:Iyọ iṣu soda HEPES nigbagbogbo wa ninu media aworan fun awọn adanwo aworan sẹẹli laaye. Agbara ifipamọ rẹ ṣe iranlọwọ lati ṣetọju pH ti o fẹ ati idilọwọ awọn iyipada ti o le ni ipa lori ṣiṣeeṣe ati fifẹ ti awọn sẹẹli.
Awọn imọ-ẹrọ Biology Molecular: Iyọ iṣu soda HEPES jẹ oojọ ti lọpọlọpọ ni ọpọlọpọ awọn ilana imọ-jinlẹ molikula, pẹlu DNA tabi ipinya RNA, PCR, ilana DNA, ati itupalẹ amuaradagba. Agbara ifipamọ rẹ ngbanilaaye fun awọn abajade deede ati igbẹkẹle lakoko awọn ilana wọnyi.
O ṣe pataki lati ṣe akiyesi pe ohun elo kan pato ati ifọkansi ti iyọ sodium HEPES le yatọ si da lori awọn ibeere idanwo ati eto igbekalẹ ti o wa labẹ ikẹkọ. Nitorinaa, o ni imọran lati kan si awọn iwe-iwe ati awọn iṣeduro olupese fun lilo to dara julọ.
